The Effect of 5-FU and Radiation on A549 Cells In Vitro

Lee,  Myung Za; Chun,  Ha Chong; Lee,  Won Young

J Korean Soc Ther Radiol > Volume 7(1); 1989 > Article

Original Article

Journal of the Korean Society for Therapeutic Radiology 1989;7(1): 1-14.

The Effect of 5-FU and Radiation on A549 Cells In Vitro

Myung Za Lee, Ha Chong Chun, Won Young Lee

¹Department of Radiation Therapy, Hanyang University Hospital, Seoul, Korea.
²Department of Radiation Oncology, Medical College of Virginia, Richmond, Virginia, U.S.A.
³Department of Microbiology, Yonsei Medical College, Seoul, Korea.

ABSTRACT

Effects of ionizing radiation alone and combined with chemotherapy on tumor growth and it's clonal specificity Monitored by changes in distribution of chromosome number were studies in A549 cell line originated from human adenocarcinoma of the lung. Radiation (300 rad, 600 rad and 900 rad) were delivered with or without 5-FU. Forty eight hours later, 57.5% of growth inhibition of cell was Seen in cells treated with 5-FU concentration of 0.47g/ml for 24 hr exposure. Cell survival carves after radiation with and without 5-FU were made. Chromosomal analysis of cells in metaphase in control, and in cells treated with 300 rad of radiation, or 0.47g/ml of 5-FU treatment, and combined treatment of cloth were 77ne to examine the changes in ploidy and number of chromosome. Radiation combined with 5-FU enhanced growth inhibition of A549 cells. However, no evidence of synergegic effects in growth inhibition was observed in the cells treated with the combination therapy. Pattern of chromosomal distribution of survived cells were shifted from hyperploidy to hypoploidy by single dose of radiation(300 rad). As radiation dose increased a large number of hypoploidy cells were observed. Following treatment of cells with 5-FU, chomosomal distribution of survived cells were also shifted to hypodiploidy, which were seen in cells treated with radiation. The cell treated with 5-FU and followed by radiation within 24 hrs had cell with increased number of hypodiploidy cells. Almost same type of chromosomal changes were reproduced in cells treated with combined treatment with radiation and 5-FU. Minor differences were that cells with fewer number of chromosome were more frequent in cells treated with combined therapy. Further increase in cells of hypoploidy(93%) having 1~10 chromosome were induced by additional radiation. Therefore, the enhanced therapeutic effect of 5-FU combined with radiation of A549 cells appeared to be additive rather than synergistic.

Key Words: 5-FU, A549 cell, Ploidy, Combined radiation and chemotherapy, Heterogeneity