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J Korean Soc Ther Radiol Oncol > Volume 28(2); 2010 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2010;28(2): 91-98. doi: https://doi.org/10.3857/jkstro.2010.28.2.91
The Effect of Troglitazone on Thermal Sensitivity in Uterine Cervix Cancer Cells
Ji Hye Lee, Won Dong Kim, Jae Ran Yu, Woo Yoon Park
1Department of Radiation Oncology, Chungbuk National University College of Medicine, Cheongju, Korea. wynpark@chungbuk.ac.kr
2Department of Environmental and Tropical Medicine, Konkuk University College of Medicine, Chungju, Korea.
Troglitazone (TRO), a PPAR-gamma agonist, can reduce heat shock protein (HSP) 70 and increase the antioxidant enzymes, such as superoxide dismutase (SOD) and catalase, which might affect thermal sensitivity. Here, we investigated whether TRO modifies thermal sensitivity in uterine cervical cancer cells, which is most commonly treated by hyperthermia (HT).
HeLa cells were treated with 5microM TRO for 24 hours before HT at 42degrees C for 1 hour. Cell survival was analyzed by clonogenic assay. The expression of HSPs was analyzed by Western blot. SOD and catalase activity was measured and reactive oxygen species (ROS) was measured using 2',7'-dichlorofluorescin diacetate and dihydroethidium.
The decreased cell survival by HT was increased by preincubation with TRO before HT. Expression of HSP 70 was increased by HT however, it was not decreased by preincubation with TRO before HT. The decreased Bcl-2 expression by HT was increased by preincubation with TRO. SOD and catalase activity was increased by 1.2 and 1.3 times,respectively with TRO. Increased ROS by HT was decreased by preincubation with TRO.
TRO decreases thermal sensitivity through increased SOD and catalase activity, as well as scavenging ROS in HeLa cells.
Key Words: Troglitazone, Hyperthermia, Reactive oxygen species
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