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J Korean Soc Ther Radiol Oncol > Volume 26(3); 2008 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2008;26(3): 166-172. doi: https://doi.org/10.3857/jkstro.2008.26.3.166
The Use of MTT Assay, In Vitro and Ex Vivo, to Predict the adiosensitivity of Colorectal Cancer
Ji Eun Kim, Mi Sook Kim, Chang Mo Kang, Jong Il Kim, Hye Kyung Shin, Chul Won Choi, Young Seok Seo, Young Hoon Ji
1Laboratory of Radiation Treatment Research, Korea Institute of radiological and Medical Sciences, Korea.
2Department of Radiation Oncology, Korea Institute of radiological and Medical Sciences, Korea. mskim@kcch.re.kr
3Laboratory of Cytogenetics and Tissue Regeneration, Korea Institute of radiological and Medical Sciences, Korea.
4Department of Food and Microbial Technology, Seoul Women's University, Seoul, Korea.
ABSTRACT
PURPOSE:
The measurement of radiosensitivity of individuals is useful in radiation therapy. Unfortunately, the measurement of radiation survival using a clonogenic assay, which is the established standard, can be difficult and time consuming. The aim of this study is to compare radiosensitivity results obtained from the MTT and clonogenic assays, and to evaluate whether the MTT assay can be used on clinical specimens.
MATERIALS AND METHODS:
HCT-8, LoVo, CT-26, and WiDr were the colon cancer cell lines used for this study. The clonogenic assay was performed to obtain the cell survival curves and surviving fractions at a dose of 2 Gy (SF2) as the standard technique for radiosensitivity. Also, the MTT assay was performed for each of the cell lines (in vitro). To simulate clinical specimens, the cell lines were inoculated into nude mice, removed when the tumors reached 1 cm in diameter, and chopped. Next, the tumors were subjected to the same process involved with the MTT assay in vitro. The inhibition rates (IR) of 10 Gy or 20 Gy of irradiation for in vitro and ex vivo were calculated based on the optical density of the MTT assay, respectively.
RESULTS:
According to SF2 and the cell survival curve, the HCT-8 and WiDr cell lines were more resistant to radiation than LoVo and CT-26 (p<0.05). The IR was measured by in vitro. The MTT assay IR was 17.3%, 21%, 30% and 56.5% for the WiDr, HCT-8, LoVo and CT-26 cell lines, respectively. In addition, the IR measured ex vivo by the MTT assay was 23.5%, 26%, 38% and 53% in the HCT-8, WiDr, LoVo and CT-26 tumors, respectively.
CONCLUSION:
The radiosensitivity measured by the MTT assay was correlated with the measures obtained from the clonogenic assay. This result highlights the possibility that the MTT assay could be used in clinical specimens for individual radiosensitivity assays.
Key Words: Clonogenic assay, MTT assay, Radiosensitivity
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