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J Korean Soc Ther Radiol Oncol > Volume 24(3); 2006 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2006;24(3): 179-184.
Effects of Recombinant Human Epidermal Growth Factor on the Proliferation and Radiation Survival of Human Fibroblast Cell Lines in Vitro
Hyun Sook Kim, Ki Mun Kang, Sang wook Lee, Jae Boem Na, Gyu Young Chai
1Department of Radiology, Gyeongsang National University, College of Medicine, Jinju, Korea.
2Department of Radiation Oncology, Gyeongsang National University College of Medicine, Jinju, Korea. cgyinj@dreamwiz.com
3Department of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
To explore the effect of recombinant human EGF on the proliferation and survival of human fibroblast cell lines following irradiation.
Fibroblast was originated human skin and primary cultured. The trypan blue stain assay and MTT assay were used to study the proliferative effects of EGF on human fibroblast cell lines in vitro. An incubation of fibroblasts with rhEGF for 24 hours immediately after irradiation was counted everyday. Cell cycle distributions were analyzed by FACS analysis.
Number of fibroblast was significantly more increased rhEGF (1.0 nM, 10 nM, 100 nM, 1,000 nM) treated cell than control after 8 Gy irradiation. Most effective dose of rhEGF was at 160 nM. These survival differences were maintained at 1 week later. Proportion of S phase was significantly increased on rhEGF treated cells.
rhEGF cause increased fibroblast proliferation following irradiation. We expect that rhEGF was effective for radiation induced wound healing.
Key Words: Recombinant human epidermal growth factor, Radiation, Fibroblast cell
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