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J Korean Soc Ther Radiol Oncol > Volume 22(4); 2004 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2004;22(4): 298-306.
Proteomics of Protein Expression Profiling in Tissues with Different Radiosensitivity
Jeung Hee An, Jiyoung Kim, Jinsil Seong
1Department of Radiation Oncology, Yonsei University College of Medicine, Korea. jsseong@yumc.yonsei.ac.kr
2Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine Seoul, Korea.
The purpose of this study was to identify Radiosensitivity of proteins in tissues with different radiosensitivity.
C3H/HeJ mice were exposed to 10 Gy. The mice were sacrifiud 8 hrs after radiation. Their spleen and liver tissues were collected and analyzed histologicaly for apoptosis. The expressions of radiosusceptibility protein were analyzed by 2-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
The peak of apoptosis levels were 35.3+/-1.7% in spleen and 0.6+/-0.2% in liver at 8 hrs after radiation. Liver, radioresistant tissues, showed that the levels of ROS metabolism related to proteins such as cytochromm c, glutathione S transferase, NADH dehydrogenase, riken cDNA and peroxiredoxin VI increased after radiation. The expression of cytochrome c increased significantly in spleen and liver tissues after radiation. In spleen, radiosensitivity tissue, the identified proteins showed a significantly quantitative alteration following radiation. It was categorized as signal transduction, apoptosis, cytokine, Ca signal related protein, stress-related protein, cytoskeletal regulation, ROS metabolism, and others.
Differences of radiation-induced apoptosis by tissues specifted were coupled with the induction of related radiosensitivity and radioresistant proteins. The result suggests that apoptosis relate protein and redox proteins play important roles in this radiosusceptibility.
Key Words: Radiation, Proteomics, Apoptosis, Radiosusceptibility
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