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J Korean Soc Ther Radiol Oncol > Volume 19(3); 2001 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2001;19(3): 245-251.
Smad6 Gene and Suppression of Radiation-Induced Apoptosis by Genistein in K562 cells
Soo Jin Jeong, Young Hee Jin, Yeo Jin Yoo, Chang Ho Do, Min Ho Jeong, Gi Yeong Huh, Hye Ran Bae, Kwang Mo Yang, Chang Woo Moon, Sin Geun Oh, Won Joo Hur, Hyung Sik Lee
1Department of Radiation Oncology, College of Medicine, Dong-A University, Pusan, Korea.
2Institute of Medical Science, College of Medicine, Dong-A University, Pusan, Korea.
ABSTRACT
PURPOSE:
The genes involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated.
MATERIALS AND METHODS:
K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. Forx-ray irradiation and drug treatment, cultures were prepared at 2x105 cells/mL. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA). Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at 37degreesC for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined.
RESULTS:
Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis.
CONCLUSION:
We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.
Key Words: PTK inhibitors, Oncogene expression, Radiation-induced apoptosis, Smad6
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