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J Korean Soc Ther Radiol Oncol > Volume 19(2); 2001 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2001;19(2): 146-152.
Regulation of Apoptosis and Cell Cycle in Irradiated Mouse Brain
Won Yong Oh, Mi Hee Song, Eun Ji Chung, Jin Sil Seong, Chang Ok Suh
1Department of Therapeutic Radiology, National Medical Center.
2Department of Radiation Oncology, Yonsei University, College of Medicine, Yonsei Cancer Center, Seoul, Korea.
ABSTRACT
PURPOSE:
To investigate the regulation of apoptosis and cell cycle in mouse brain irradiation.
MATERIALS AND METHODS:
8-week old male mice, C57B1/6J were given whole body gamma-radiation with a single dose of 25 Gy using Cobalt 60 irradiator. At different times 1, 2, 4, 8 and 24hr after irradiation, mice were killed and brain tissues were collected. Apoptotic cells were scored by TUNEL assay. Expression of p53, Bcl-2, and Bax and cell cycle regulating molecules; cyclins B1, D1, E and cdk2, cdk4, p34cdc2 were analysed by Western blotting. Cell cycle was analysed by Flow cytometry.
RESULTS:
The peak of radiation induced apoptosis is shown at 8 hour after radiation. With a single 25 Gy irradiation, the peak of apoptotic index in C57B1/6J is 24.0+/-0.25 (p<0.05) at 8 hour after radiation. Radiation upregulated the expression of p53/tubulin, Bax/tubulin, and Bcl-2/tubulin with 1.3, 1.1 and 1.45 fold increase, respectively were shown at the peak level at 8 hour after radiation. The levels of cell cycle regulating molecules after radiation are not changed significantly except cyclin D1 with 1.3 fold increase. Fractions of Go-G1, G2-M and S phase in the cell cycle does not specific changes by time.
CONCLUSIONS:
In mouse brain tissue, radiation induced apoptosis is particularly shown in a specific area, subependyma. These results and lack of radiation induced changes in cell cycle offer better understanding of radiation response of normal brain tissue.
Key Words: Mouse brain(cerebrum) irradiation, Apoptosis, Cell cycle
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