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J Korean Soc Ther Radiol Oncol > Volume 18(4); 2000 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2000;18(4): 321-328.
Radiation Effect on NO, NOS and TGF-beta Expressions In Rat Lung
Young Taek Oh, Kwang Joo Park, Hoon Jong Kil, Mahn Joon Ha, Mison Chun, Seung Hee Kang, Seong Eun Park, Sei Kyung Chang
1Department of Radiation Oncology, School of Medicine, Ajou University, Suwon, Korea.
2Department of Pulmonary Medicine, School of Medicine, Ajou University, Suwon, Korea.
3Institute for Medical Sciences, School of Medicine, Ajou University, Suwon, Korea.
4Department of Radiation Oncology, Eulji Medical College, Daejon, Korea.
ABSTRACT
PURPOSE:
NOS2 induce NO production and NO activate TGF-beta. The TGF-beta is a inhibitor of NOS2. If this negative feedback mechanism operating in radiation pneumonitis model, NOS2 inhibitor may play a role in TGF-beta suppression. We planned this study to evaluate the expression patterns of NO, NOS2 and TGF-beta in vivo radiation pneumonitis model.
MATERIALS AND METHODS:
Sixty sprague-Dawley rat were irradiated 5 Gy or 20 Gy. They were sacrificed 3, 7, 14, 28 and 56 days after irradiation. During sacrifice, we performed broncho-alveolar lavage (BAL). The BAL fluids were centrifuged and supernatents were used for measure NO and TGF-beta, and the cells were used for RT-PCR.
RESULTS:
After 5 Gy of radiation, NO in BAL fluid increased at 28 days in both lung and TGF-beta in left lung at 56 days. NO increased in BAL fluid at 28 days in both lung after irradiation and TGF-beta in right lung at 28-56 days after 20 Gy of radiation. After 5 Gy of radiation, NOS2 expression was increased in right lung at 14 days, in both lung at 28 days and in left lung at 56 days. TGF-beta expression was reduced in both lung at 28 days and increased in left lung at 56 days.
CONCLUSIONS:
The proposed feedback mechanism of NO, NOS2 and TGF-beta was operated in vivo radiation pneumonitis model. At 56 days, however, NOS2 and TGF-beta expressed concurrently in left lung after 5 Gy and in both lung after 20 Gy of radiation.
Key Words: Radiation pneumonitis, NO, NOS2, TGF-beta, Radiation
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