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J Korean Soc Ther Radiol Oncol > Volume 18(3); 2000 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2000;18(3): 205-213.
The Relationship between Radiation-Induced Apoptosis and theExpression of Cytokines in the Rat's Liver
Eung Joo An, Kyung Ja Lee, Chung Sik Rhee
1Department of Diagnostic Radiology, Pundang Jesaeng General Hospital, Korea.
2Department of Radiation Oncology, College of Medicine, Ewha Womans University, Seoul, Korea.
3Diagnostic Radiology, College of Medicine, Ewha Womans University, Seoul, Korea.
ABSTRACT
PURPOSE:
To determine the role of cytokines in the apoptosis of rat's liver following irradiation.
MATERIALS AND METHODS:
Sprague-Dawley rats were irradiated to entire body with a single dose of 8 Gy. The rats were divided into 5 groups according to the sacrifice day after irradiation. The liver and blood after 1, 3, 5, 7, and 14 days irradiation were sampled for evaluation of mechanism of apoptosis and role of cytokine in relation to radiation-induced tissue damage. The study was composed of microscopic evaluation of liver tissue, in situ detection method for apoptosis, immunohistochemical stain of IL-1, IL-4, IL-6 and TNF, bioassay and radioimmunoassay of IL-6 in liver tissue and blood.
RESULTS:
Radiation-induced liver damage was noted from first day of radiation, and most severe parenchymal damage associated with infiltration of chronic inflammatory cells was seen in the groups of 5 days after radiation. A number of apoptosis were observed 1 day after radiation on both light microscope and in situ method. Afterwards, the number of apoptosis was gradually diminished. On immunohistochemical study, IL-1 and TNF were expressed 1, 3 days after radiation, but not expressed after that. IL-4 was not expressed in the entire groups. IL-6 was expressed with strong positivity in 1, 3 days after radiation. Bioassay and RIA of IL-6 in liver tissue and blood showed the highest value in 1 day after radiation, and the value is diminished after then.
CONCLUSION:
Apoptosis seemed to be the important mechanism of radiation-induced liver damage, and is possibly induced by the release of cytokines, such as IL-1, IL-6, TNF in view the simultaneously increased appearance of apoptosis and cytokines.
Key Words: Radiation, Liver, Apoptosis, Cytokine
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