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The Journal of the Korean Society for Therapeutic Radiology and Oncology 2000;18(3): 200-204. |
Cytotoxic Effect of X-irradiation of Mouse Tumor Cells in the Presence of Korean Ginseng Extract |
Hyoung Cheol Kwon, Jin Ki Kim, Jung Soo Kim, Dong Seong Choi |
1Department of Therapeutic Radiology and Oncology, Institute for Medical Sciences, Medical School, Chonbuk National University, Korea. 2Division of Bioscience and Biotechnology, Woo Suck University, Korea. |
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ABSTRACT |
PURPOSE: We already reported the results that aqueous extract of Korean ginseng roots showed a marked cytotoxicity. In this study, we investigated whether combined ginseng product with X-irradiation increase the cytotoxicity of tumor cells than X-irradiation or not.
MATERIALS AND METHODS: Fifty gram of Korean ginseng powder mixed with 1 L of distilled water was extracted with reflux flask under condition of 100 degrees C for 5 hrs. This aquaous ginseng extract was filtered, centrifuged and then was freezed under condition of -90degrees C for 16-18 hrs.
The freezing extract was dried with freeze drier, and then diluted. X-irradiation was given to tumor cells by 6 MeV linear accelerator. The cytotoxicity of ginseng in vitro was evaluated from its ability to reduce the clonogenecity of fibrosarcoma (FSa II) cells. In X-irradiation alone group, each 2, 4, 6 and 8 Gy was given to tumor cells. In X-irradiation with ginseng group, 0.2 mg/mL of ginseng extract was exposed to tumor cells for 1 hour before X-irradiation.
RESULTS: The yield for 50 g of ginseng extract which was treated with freezing drier was 3.13 g (6.3%). Cytotoxicity in vitro was measured as survival fraction which was judged from the curve, at ginseng concentration of 0.001, 0.01, 0.1 and 1 mg/mL were 0.89+/-0.04, 0.86+/-0.06, 0.73+/-0.01 and 0.09+/-0.02, respectively. Survival fraction at X-irradiation alone of 2, 4, 6 and 8 Gy were 0.81+/-0.07, 0.42+/-0.08, 0.15+/-0.02, 0.03+/-0.01, respectively. But, survival fraction in combined group of X-irradiation and ginseng (0.2mg/mL) at each same radiation dose were 0.28+/-0.01, 0.18+/-0.03, 0.08+/-0.02, 0.006+/-0.002, respectively ( p<0.05).
CONCLUSION: The yield for ginseng extract which was treated with freezing drier was 6.3%. Cytotoxicity of Fsa II in combined ginseng with X-irradiation group was increased than that of X-irradition alone group, and its enhancing effect seemed to be added. |
Key Words:
Korean ginseng, X-irradiation, Cytotoxicity, Combined effect |
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