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J Korean Soc Ther Radiol Oncol > Volume 16(2); 1998 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 1998;16(2): 99-106.
Correlation Between the Parameters of Radiosensitivity in Human Cancer Cell Lines
Woo Yoon Park, Won Dong Kim, Kyung Soo Min
1Department of Therapeutic Radiology, College of Medicine, Chungbuk National University, Cheongju, Korea.
2Department of Neurosurgery, College of Medicine, Chungbuk National University, Cheongju, Korea.
We conducted clonogenic assay using human cancer cell lines (MKN-45, PC-14, Y-79, HeLa) to investigate a correlation between the parameters of radiosensitivity.
Human cancer cell lines were irradiated with single doses of 1, 2, 3, 5, 7 and 10Gy for the study of radiosensitivity and sublethal damage repair capacity was assessed with two fractions of 5Gy separated with a time interval of 0, 1, 2, 3, 4, 6 and 24 hours. Surviving fraction was assessed with clonogenic assay using Sperman-K rber method and mathematical analysis of survival curves was done with linear-quadratic (LQ), multitarget-single hit (MS) model and mean inactivation dose (D).
Surviving fractions at 2Gy (SF2) were variable among the cell lines, ranged from 0.174 to 0.85. The SF2 of Y-79 was lowest and that of PC-14 was highest (p<0.05, t-test). LQ model analysis showed that the values of alpha for Y-79, MKN-45, HeLa and PC-14 were 0.603, 0.356, 0.275 and 0.102 respectively, and those of beta were 0.005, 0.016, 0.025 and 0.027 respectively. Fitting to MS model showed that the values of Do for Y-79, MKN-45, HeLa and PC-14 were 1.59, 1.84, 1.88 and 2.52 respectively, and those of n were 0.97, 1.46, 1.52 and 1.69 respectively. The s calculated by Gauss-Laguerre method were 1.62, 2.37, 2.61 and 3.95 respectively. So the SF2 was significantly correlated with alpha, Do and D. Their Pearson correlation coefficiencics were -0.953 and 0.993, 0.999 respectively (p<0.05). Sublethal damage repair was saturated around 4 hours and recovery ratios (RR) at plateau phase ranged from 2 to 3.79. But RR was not correlated with SF2, alpha, beta, Do, D.
The intrinsic radiosensitivity was very different among the tested human cell lines. Y-79 was the most sensitive and PC-14 was the least sensitive. SF2 was well correlated with alpha, Do, and D. RR was high for MKN-45 and HeLa but had nothing to do with radiosensitivity parameters. These basic parameters can be used as baseline data for various in vitro radiobiological experiments.
Key Words: Radiosensitivity, Sublethal damage repair, MKN-45, PC-14
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