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J Korean Soc Ther Radiol > Volume 12(1); 1994 > Article
Journal of the Korean Society for Therapeutic Radiology 1994;12(1): 27-32.
In Vitro Intrinsic Radiosensitivity Of Human Squamous Cell Carcinoma in Primary Culture
Eun Kyung Choi, Kwang Mo Yang, Byong Yong Yi, Hyesook Chang, Sang Yoon Kim, Joo Hyun Nam, Eun Sil Yu, Inchul Lee
1Department of Therapeutic Radiology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
2Department of Otolaryngology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
3Department of Obstetrics and Gynecology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
4Department of Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
ABSTRACT
There are a number of reports suggesting that there may be a correlation between the clinical response to radiotherapy in various tumors and the clonogenic survival of cell lines derived from these tumor following exposure to 2 Gy (SF2). Authors conducted this study to determine SF2 for cells in primary culture from surgical specimens. The tumor tissues with squamous cell carcinoma of uterine cervix and head and neck were obtained. The tumor tissues were disaggregated to single cells by incubating with collagenase typeIV for 2 hours with constant stirring. Single cell suspensions were inoculated in four 24-well plates precoated with cell adhesive matrix. After 24 hours of incubation at 37degree C, rows of four wells were then irradiated, consisting of control set and five other sets each receiving doses of 1,2,3,4, and 6 Gy. After incubation for a total of 13 days, the cultures were stained with crystal violet determine whether cell growth was of epithelial origin, immunocytochemical staining with a mixture of cytokeratin and epithelial monoclonal antibodies were performed on cell cultures. During the period of this study, we received 5 squamous cell carcinoma specimens of head and neck and 20 of uterine cervical carcinoma. Of these, 15 yielded enough cells for radiosensitivity testing. This resulted an overall success rate of 60%. The mean SF2 value for 15 tumors was 0.55+/-0.17 ranging from 0.20 to 0.79. These results indicate that there is a broad range of sensitivities to radiation in same histologic type. So with a large patient population, we plan to determine whether a different SF2 value is associated with tumors that are controlled with radiotherapy than those that are not.
Key Words: Survival at 2Gy, Primary culture, Squamous cell carcinoma, Head and neck cancer, Cervix cancer
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