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J Korean Soc Ther Radiol > Volume 11(1); 1993 > Article
Journal of the Korean Society for Therapeutic Radiology 1993;11(1): 35-42.
Frequency of Micronuclei in Lymphocytes Following Gamma and Fast-neutron Irradiations
Sung Ho Kim, Chul Koo Cho, Tae Hwan Kim, In Yong Chung, Seong Yul Yoo, Kyoung Hwan Koh, Hyong Geun Yun
1Department of Radiotherapy, Korea Cancer Center Hospital, Seoul, Korea.
2Department of Laboratory of Radiation Medicine, Korea Cancer Center Hospital, Seoul, Korea.
ABSTRACT
The dose response of the number of micronuclei in cytokinesis-blocked (CB) lymphocytes after in vitro irradiation with -rays and neutrons in the 5 dose ranges was studied for a heterogeneous population of 4 donors. One thousand binucleated cells were systematically scored for micronuclei. Measurements performed after irradiation showed a dose-dependent increase in micronuclei(MN) frequency in each of the doctors studied. The dose-response curves were analyzed by a linear-quadratic model, frequencies per 1000 CB cells were (0.31+/-0.049) D+(0.0022+/-0.0002) D2+(13.19+/-1.854) (r2=1.000, X2=0.7074, p=0.95) following irradiation, and (0.99+/-0.528) D+(0.0093+/-0.0047) D2+(13.31+/-7.309) (r2=0.996, X2=7.6834, p=0.11) following neutrons irradiation (D is irradiation dose in cGy). The relative biological effectiveness (RBE) of neutrons compared with -rays was estimated by best fitting linear-quadratic model. In the micronuclei frequency between 0.05 and 0.8 per cell, the RBE of neutrons was 2.37+/-0.17. Since the MN assay is simple and rapid, it may be a good tool for evaluating the y-ray and neutron response.
Key Words: Micronuclei, Gamma and neutron, Biological dosimetry, Cytokinesis blocked cell, Relative biological effectiveness
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