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J Korean Soc Ther Radiol Oncol > Volume 27(4); 2009 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2009;27(4): 218-227. doi: https://doi.org/10.3857/jkstro.2009.27.4.218
Different Expressions of HIF-1alpha, Bcl-2 and Baxin DU145 Prostate Cancer Cells Transplanted in Nude Mouse between X-Ray and Neutron Irradiation
Moonkyoo Kong, Jin Oh Kang, Sang Ki Kim, Dong Oh Shin, Seo Hyun Park, Chang Ju Kim, Hyun Kyung Chang
1Department of Radiation Oncology, Kyung Hee University School of Medicine, Seoul, Korea. kangjino@paran.com
2Department of Physiology, Kyung Hee University School of Medicine, Seoul, Korea.
To investigate the radiobiologic effects of neutron and X-ray irradiation on DU-145 prostate carcinoma cells by identifying the differences of HIF-1alpha expression and apoptosis.
Nude mice were injected with the human prostate cancer cell line, DU-145, and then irradiated with 2 Gy and 10 Gy X-rays, or 0.6 Gy and 3.3 Gy neutrons, respectively. The mice were sacrificed at 24 hours and 120 hours after irradiation. The expression levels of HIF-1alpha, Bcl-2 and Bax were compared with immunohistochemical staining and western blotting. The apoptotic indexes were compared with the Terminal deoxynucleotidyl biotin-dUTP nick and labeling (TUNEL) assay.
At day 1, HIF-1alpha and Bcl-2 expression decreased, while Bax expression and the number of TUNEL positive cells increased in neutron irradiated groups for the control and X-ray irradiated groups. The Bcl-2/Bax ratio was significantly lower in the neutron irradiated groups regardless of dose (p=0.001). The same pattern of the differences in the expressions of the HIF-1alpha, Bcl-2, Bax, Bcl-2/Bax ratio, and apoptotic indexes were indentified at day 5. HIF-1alpha expression was related with Bcl-2 (p=0.031), Bax (p=0.037) expressions and the apoptotic indexes (p=0.016) at day 5.
Neutron irradiation showed a decrease in HIF-1alpha, Bcl-2 expression, and Bcl-2/Bax ratio, but increased Bax expression regardless of dose. This study suggests that the differences radiobiological responses between photon and neutron irradiation may be related to different HIF-1alpha expression and subsequent apoptotic protein expressions.
Key Words: HIF-1alpha, Bcl-2, Bax, Neutron, Prostate cancer
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