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J Korean Soc Ther Radiol > Volume 9(1); 1991 > Article
Journal of the Korean Society for Therapeutic Radiology 1991;9(1): 1-6.
Effects of 2-Deoxy-D-Glucose on Metabolic Status, Proliferative Capacity and Growth Rate of FSall Tumor: Observations made by In Vivo 31P-Nuclear Magnetic Resonance Spectroscopy and Flow Cytometry
Hyesook Chang, Eun Kyung Choi, Jeong Gil Cho, Tae Hwan Lim, Tae Keun Lee, Yun Yi, Young Joo Cho, Gon Sup Kim
1Department of Therapeutic Radiology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
2NMR Division, Asan Institute for Life Sciences, Seoul, Korea.
3Department of Medical Electronics, Korea University, Seoul, Korea.
4Cell Biology Division, Asan Institute for Life Sciences, Seoul, Korea,
5Animal Research Division, Asan Institue for Life Sciences, Seoul, Korea.
ABSTRACT
The effect of 2-deoxy-d-glucose (2-DDG) on C3H mouse fibrosarcoma (FSall) was studied. Metabolic status, especially for energy metabolism, was studied using in vivo 31P-MRS, proliferative capacity was observed on flow cytometry (FC) and growth rate was measured after transplantation of 106 viable tumor cells in the dorsum of foot of C3Hf/Sed mice. One gram of 2-DDG per kg of body weight was injected intraperitoneally on 12th day of implantation. Average tumor size on 12th day of implantation was 250mm3. Growth rate of FSall tumor was measured by tumor doubling time between tumor age 5-12 days was 0.84 days with slope 0.828 and tumor doubling time between tumor age 13-28 days was 3.2 days with slope 0.218 in control group. After 2-DDG injection, tumor doubling time was elongated to 5.1 days with slope 0.136. The effect of 2-DDG studied in vivo 31P-MRS suggested that the increase of phosphomonoester (PME) and inorganic phosphate (Pi) by increasing size of tumor, slowed down after 2-DDG injection. Flow cytometry showed significantly increased S-phase and G2+M phase fraction suggesting increased proliferative capacity of tumor cells in the presence of 2-DDG. Authors observed an interesting effect 2-DDG on FSall tumor and attempt to utilize as an adjunct for radiotherapy.
Key Words: 2-DDG, 31P-MRS, FSall tumor
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