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J Korean Soc Ther Radiol Oncol > Volume 28(3); 2010 > Article
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2010;28(3): 147-154. doi: https://doi.org/10.3857/jkstro.2010.28.3.147
Combination Effect of Nimotuzumab with Radiation in Colorectal Cancer Cells
Hye Kyung Shin, Mi Sook Kim, Jae Hoon Jeong
1Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul, Korea. jeongj@kirams.re.kr
2Department of Radiation Oncology, Korea Institute of Radiological and Medical Sciences, Seoul, Korea. mskim@kcch.re.kr
ABSTRACT
PURPOSE:
To investigate the radiosensitizing effect of the selective epidermal growth factor receptor (EGFR) inhibitor nimotuzumab in human colorectal cancer cell lines.
MATERIALS AND METHODS:
Four human colorectal cancer cell lines, HCT-8, LoVo, WiDr, and HCT-116 were treated with nimotuzumab and/or radiation. The effects on cell proliferation, viability, and cell cycle progression were measured by MTT, clonogenic survival assay, flow cytometry, and Western blot.
RESULTS:
An immunoblot analysis revealed that EGFR phosphorylation was inhibited by nimotuzumab in colorectal cancer cell lines. Under these experimental conditions, pre-treatment with nimotuzumab increased radiosensitivity of colorectal cancer cell lines, except for cell line HCT-116. However, cell proliferation or cell cycle progression was not affected by the addition of nimotuzumab, irrespective of irradiation.
CONCLUSION:
Nimotuzumab enhanced the radiosensitivity of colorectal cancer cells in vitro by inhibiting EGFR-mediated cell survival signaling. This study provided a rationale for the clinical application of the selective EGFR inhibitor, nimotuzumab in combination with radiation in colorectal cancer cells.
Key Words: Colorectal cancer, EGFR, Nimotuzumab, Radiation, Combined therapy
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